Journal
PLANT CELL TISSUE AND ORGAN CULTURE
Volume 110, Issue 2, Pages 261-273Publisher
SPRINGER
DOI: 10.1007/s11240-012-0148-3
Keywords
D-type cyclins; Arath;CYCD2;1; Leaf rolling; Plant architecture; Transgenic cotton
Funding
- National Natural Science Foundation of China [30810103911]
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The D-class cyclin genes play key roles in controlling the cell cycle in plant development. To evaluate the effects of D-type cyclins on plant architecture, ectopic expression of the Arabidopsis cyclinD2;1 (Arath;CYCD2;1) gene driven by the CaMV35S promoter was investigated in cotton via Agrobacterium-mediated transformation. Northern blot showed the cyclinD2;1 gene was highly expressed in transgenic cotton plants. Phenotype investigation showed that overexpression of Arath;CYCD2;1 led to obvious leaf architecture change: the leaf epidermis of transgenic plants consist of more small cells compared to the wild-type. Mesophyll cells in the inner layers of Arath;CYCD2;1 plants were organized more loosely than those in the inner layers of wild-type plants. Moreover, transgenic plants had darker leaves, more chlorophyll, and a higher rate of photosynthesis than wild-type plants in the field. Tissue cultures indicated that the overexpression of Arath;CYCD2;1 promoted callus formation in the absence of exogenous auxin, but inhibited cell differentiation. The qRT-PCR revealed that several cell cycle-associated genes, particularly the transcript levels of GhRBR and GhCYCD3;1 were regulated by the Arath; CYCD2;1 insertion. The results implied that the cotton plant architecture or cell culture characters could be regulated by ectopic expression of the Arabidopsis cyclinD2;1 gene.
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