Journal
PLANT CELL TISSUE AND ORGAN CULTURE
Volume 109, Issue 1, Pages 51-59Publisher
SPRINGER
DOI: 10.1007/s11240-011-0072-y
Keywords
Chalcone synthase gene; Expression analysis; Flavonoids; In vitro culture; Juglans regia; Walnut
Funding
- University of Tehran, College of Science
Ask authors/readers for more resources
Adventitious rooting of proliferating shoots is controlled by many physical and chemical factors including phenolic and flavonoid compounds. Chalcone synthase (CHS) is an important enzyme in allocating significant amounts of carbon in the phenypropanoid pathway into the biosynthesis of flavonoid compounds. In this study, the expression of CHS gene during rhizogenesis of in vitro-grown shoots of two genotypes of walnut, 'Sunland' and 'Howard', was investigated using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). The amounts of flavonoids, flavonols, proanthocyanidins and auxin in these cultures were also determined. Patterns of CHS gene expression in different genotypes of shoots undergoing rhizogenesis were associated with observed flavonoids content. Similar observations were also noted for flavonol and proanthocyanidin contents in 'Howard' walnut. Overall, a high frequency of rhizogenesis was accompanied with low flavonoid content in shoots. These observed relationships suggested that chalcone synthase is involved in control of flavonoid biosynthesis and in promoting rhizogenesis in walnut microshoots.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available