Journal
PLANT CELL TISSUE AND ORGAN CULTURE
Volume 105, Issue 1, Pages 73-78Publisher
SPRINGER
DOI: 10.1007/s11240-010-9843-0
Keywords
Bromeliad; Aechmea fasciata; Floral organ segments; Micropropagation; Acclimatization
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Although suckers and seedlings can be used for the propagation of bromeliads, the low number of propagules and cross-variation limit their uniformity and mass cultivation. In this study, high-efficiency shoot organogenesis and plant regeneration were achieved on callus derived from petal and ovary explants of Aechmea fasciata (Bromeliaceae). Calluses were induced on half-strength Murashige and Skoog inorganic salts (1/2MS) supplemented with 1.0-1.5 mg l(-1) 2,4-dichlorophenoxyacetic acid in combination with 1.0 or 0.5 mg l(-1) alpha-naphthaleneacetic acid (NAA), and shoots regenerated after transfer to 1/2MS basal medium containing the combination of 1.0 mg l(-1) NAA + 0.5 mg l(-1) 1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea. Those plantlets grown under a middle light intensity (50 mu mol m(-2) s(-1)) showed a dramatic increase in survival percentage (up to 95%) and the maximum number of newly developing roots. The plantlets that were transplanted onto pots were successfully grown in the greenhouse.
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