Journal
PLANT CELL TISSUE AND ORGAN CULTURE
Volume 103, Issue 3, Pages 325-332Publisher
SPRINGER
DOI: 10.1007/s11240-010-9783-8
Keywords
Dendrocalamus hamiltonii; Zygotic embryo; Embryogenesis; Organogenesis
Funding
- Science and Technology Department of Zhejiang Province, China [2006C12008]
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In this study, mature zygotic embryos, plant growth regulators, and various media were tested with the aim of developing an efficient regeneration system for plantlets of the bamboo species Dendrocalamus hamiltonii. Callus formation was induced in explants cultured in Murashige and Skoog (MS) medium supplemented with 1.0-3.0 mg/l 2,4-dichlorophenoxyacetic acid. Optimal shoot differentiation and subsequent shoot growth were also obtained in MS medium supplemented with 2 mg/l benzyladenine, 1 mg/l kinetin, and 1 mg/l naphthaleneacetic acid. Root induction was enhanced by the addition of 5 mg/l indole-3-butyric acid to the culture medium. Histological analysis revealed that both somatic embryogenesis and organogenesis were induced during callus initiation, shoot differentiation, and the development of plantlets from the mature zygotic embryos. Our data provide a useful basis for developing culture protocols for the regeneration of bamboo plants.
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