Journal
PLANT CELL TISSUE AND ORGAN CULTURE
Volume 104, Issue 2, Pages 171-179Publisher
SPRINGER
DOI: 10.1007/s11240-010-9814-5
Keywords
Ammonical nitrogen; EM medium; Sucrose concentration; Rosa hybrida; Anthocyanin pigments
Funding
- Indian Council of Agricultural Research, New Delhi
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The influence of varied concentrations of sucrose and ammonical (NH (4) (+) ) nitrogen on in vitro induction and expression of anthocyanin pigments from Rosa hybrida cv. 'Pusa Ajay' was investigated. Of two explants (petal and leaf discs) selected and cultured under two different conditions (light and dark), leaf discs were found to be most suitable for callus initiation. Profuse and early callus induction was observed when leaf discs of rose were cultured under total dark conditions on solid Murashige and Skoog (MS) medium supplemented with 4.0 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D). Early pigment initiation, enhancement and maximum anthocyanin production from calluses were recorded when leaf discs were cultured on Euphorbia millii (EM) medium supplemented with 7% sucrose compared with calluses cultured at 4% sucrose concentration under 16/8 h (light/dark) photoperiod regime. Reducing the concentration of NH (4) (+) nitrogen in the solid MS medium led to slight improvement in anthocyanin production in rose leaf calluses.
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