Journal
PLANT CELL TISSUE AND ORGAN CULTURE
Volume 98, Issue 3, Pages 239-248Publisher
SPRINGER
DOI: 10.1007/s11240-009-9560-8
Keywords
Boron; Calcium; Physiological disorder; Plant growth regulators; Tissue culture media components
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Plant tissue culture plays an important role in the production and conservation of plant species. Its application, however, is hindered by some growth abnormalities such as shoot-tip necrosis (STN) caused by the culture conditions. This review article summarizes the literature published on the causes of in vitro STN in plants such as medium type, plant growth regulators, calcium, boron, medium additives, the culture environment, their interaction and physiological effects.
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