Journal
PLANT CELL TISSUE AND ORGAN CULTURE
Volume 97, Issue 1, Pages 91-101Publisher
SPRINGER
DOI: 10.1007/s11240-009-9505-2
Keywords
Habenaria bractescens; In vitro tuberization; Orchids; Root tuber anatomy
Funding
- FPLA'S (Fondo Para Las Americas)
- FORA (Federacion Civil de Orquideofilos de la Republica Argentina)
- CONICET
- SGCyT (Secretaria General de Ciencia y Tecnica, UNNE)
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Tuberization in many terrestrial orchids represents the most important physiological process for reproduction and survival. An in vitro controlled and reproducible tuberization and plant regeneration system was designed for Habenaria bractescens. Multinodal segments were incubated on Murashige and Skoog (MS) medium supplemented with different concentrations of N-6-benzylaminopurine (BAP) and sucrose. After 45 days, the explants developed root tubers in vitro in 8 of the 12 media assayed. MS medium with 87.6 mM sucrose plus 4.4 mu M BAP was one of the most effective for stimulating root tubers. Plants derived from in vitro root tubers were successfully transferred to the greenhouse without any acclimatization. The morphology and anatomy of the regenerated underground organs were examined to find identifying and distinguishing features. The protocol to regenerate root tubers of H. bractescens will be useful to study the basic aspects and control of tuberization and to carry out restoration programs.
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