Journal
PLANT CELL REPORTS
Volume 31, Issue 3, Pages 461-477Publisher
SPRINGER
DOI: 10.1007/s00299-011-1165-0
Keywords
Exudation; Cell culture; Hairy root; Secondary metabolite; Medium; Elicitor; Membrane permeabilization; Product recovery
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This review addresses methods of obtaining secondary metabolites from plant cell suspension and hairy root cultures and their exudates, particularly the physiological mechanisms of secondary metabolites release and trafficking. The efficiency for product recovery of metabolites can be increased by various methods, based on the principle of continuous product release into the cultivation medium. The most common methods for metabolite recovery are elicitation, influencing membrane permeability, and in situ product removal. The biosynthetic pathways can be influenced by cultivation conditions, transformation, or application of elicitors. The membrane permeability can be altered through the application of chemical or physical treatments. Product removal can be greatly increased through a two-phase system and the introduction of absorbents into the cultivation medium. In this review, we describe some improved approaches that have proven useful in these efforts.
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