Journal
PLANT CELL REPORTS
Volume 30, Issue 8, Pages 1393-1404Publisher
SPRINGER
DOI: 10.1007/s00299-011-1048-4
Keywords
Fatty acid desaturase; Peanut (Arachis hypogaea L.); Phylogenetic analysis; Quantitative real-time RT-PCR; Saccharomyces cerevisiae
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Funding
- Modern Agro-industry Technology Research System [nycytx-19]
- National High-Tech Research and Development Plan of China [2006AA10A114, 2007AA10Z189]
- National Project of Scientific and Technical Supporting Program [2008BAD97B04]
- National Natural Science Foundation of China [31000728]
- Natural Science Fund of Shangdong Province [ZR2009DQ004]
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Fatty acid desaturases are responsible for the insertion of double bonds into pre-formed fatty acid chains in reactions that require oxygen and reducing equivalents. In this study, genes for FAB2, FAD2-2, FAD6 and SLD1, were cloned from peanut (Arachis hypogaea L.). The ORFs of the four genes were 1,221, 1,152, 1,329 and 1,347 bp in length, encoding 406, 383, 442 and 448 amino acids, respectively. The predicted amino acid sequences of AhFAB2, AhFAD2-2, AhFAD6, AhSLD1 shared high sequence identity of 79, 76.2, 73.4 and 61% to the corresponding ones in Arabidopsis, respectively. Heterologous expression in yeast was used to confirm the regioselectivity and the function of AhFAD2-2 and AhFAD6. Linoleic acid (18:2), normally not present in wild-type yeast cells, was detected in transformants of these two genes. Quantitative real-time RT-PCR analysis indicated that the transcript abundances of AhFAB2 and AhFAD2-1 were higher in seed than that in other tissues examined. On the other hand, transcript of AhFAD2-2, AhFAD6 and AhSLD1 showed higher abundances in leaves. In addition, these five genes showed different expression patterns during seed development. These results indicated that the five genes may have different biochemical functions during vegetative growth and seed development.
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