Journal
PLANT CELL REPORTS
Volume 31, Issue 3, Pages 539-549Publisher
SPRINGER
DOI: 10.1007/s00299-011-1191-y
Keywords
Rice; Seed storage protein; RNA interference; Protein body; Human growth hormone
Categories
Funding
- Ministry of Education, Culture, Sports, Science, and Technology of Japan [21580417]
- Grants-in-Aid for Scientific Research [21580417] Funding Source: KAKEN
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Rice seeds are potentially useful hosts for the production of pharmaceutical proteins. However, low yields of recombinant proteins have been observed in many cases because recombinant proteins compete with endogenous storage proteins. Therefore, we attempt to suppress endogenous seed storage proteins by RNA interference (RNAi) to develop rice seeds as a more efficient protein expression system. In this study, human growth hormone (hGH) was expressed in transgenic rice seeds using an endosperm-specific promoter from a 10 kDa rice prolamin gene. In addition, an RNAi cassette for reduction of endogenous storage protein expressions was inserted into the hGH expression construct. Using this system, the expression levels of 13 kDa prolamin and glutelin were effectively suppressed and hGH polypeptides accumulated to 470 mu g/g dry weight at the maximum level in transgenic rice seeds. These results suggest that the suppression of endogenous protein gene expression by RNAi could be of great utility for increasing transgene products.
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