Journal
PLANT CELL REPORTS
Volume 30, Issue 1, Pages 27-36Publisher
SPRINGER
DOI: 10.1007/s00299-010-0937-2
Keywords
AtEXPA1; Expansin; Guard cells; Stomatal movement; Volumetric elastic modulus
Categories
Funding
- National Basic Research Program of China [2006CB100100]
- National Natural Science Foundation of China [30721062]
- 111 Project [B06003]
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Guard cell walls of stomata are highly specialized in plants. Previous research focused on the structure and anatomy of guard cell walls, but little is known about guard cell regulation during stomata movement. In this work, we investigate the possible biological role of the Arabidopsis expansin gene AtEXPA1 in stomatal opening. The AtEXPA1 promoter drove the expression of the GUS reporter gene specifically in guard cells. Light-induced stomatal opening was accelerated in 35S::AtEXPA1 lines, whereas the anti-AtEXPA1 antibody decelerated light-induced stomatal opening. The inhibition of the anti-AtEXPA1 antibody on stomatal opening was largely dependent on the environmental pH. The volumetric elastic modulus (epsilon) was measured as an indicator of changes in the cell wall. The epsilon value of guard cells in 35S::AtEXPA1 lines was smaller than in the wild types. The putative role of AtEXPA1 as controller of stomatal opening rate and its regulation are discussed.
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