Journal
PLANT CELL REPORTS
Volume 29, Issue 11, Pages 1215-1225Publisher
SPRINGER
DOI: 10.1007/s00299-010-0907-8
Keywords
Micropropagation; Disease resistance; Apple scab; Fruit quality; Shelf life; Transgenic; Cisgenic; Intragenic
Categories
Funding
- University Grants Commission, Govt of India
- CSIR
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Apple (Malus domestica Borkh.), which is a widely cultivated, important economic fruit crop with nutritive and medicinal importance, has emerged as a model horticultural crop in this post-genomic era. Apple cultivation is heavily dependent on climatic condition and is susceptible to several diseases caused by fungi, bacteria, viruses, insects, etc. Extensive research work has been carried out to standardize tissue culture protocols and utilize them in apple improvement. We review the in vitro shoot multiplication, rooting, transformation and regeneration methodologies in apple and tabulate various such protocols for easy reference. The utility and limitation of transgenesis in apple improvement have also been summarized. The concepts of marker-free plants, use of non-antibiotic resistance selectable markers, and cisgenic and intragenic approaches are highlighted. Furthermore, the limitations, current trends and future prospects of tissue culture-mediated biotechnological interventions in apple improvement are discussed.
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