Journal
PLANT CELL REPORTS
Volume 28, Issue 7, Pages 1065-1074Publisher
SPRINGER
DOI: 10.1007/s00299-009-0706-2
Keywords
Agrobacterium tumefaciens; Indica rice; Transformation; Vacuum infiltration
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Funding
- Ministry of Education of China [2002-78]
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Traditional transformation methods are complex and time consuming. It is generally difficult to transform indica rice varieties using traditional transformation methods due to their poor regeneration. In this contribution, a simple method was developed for the transformation of indica rice. In this method, the mature embryos of soaked seeds were pierced by a needle, and then soaked in the Agrobacterium inoculum under vacuum infiltration. The inoculated seeds germinated and grew to maturation (T (0)) under nonsterile conditions. The herbicide or antibiotic analysis and molecular analysis were conducted on T (0) plants. The results showed that although the efficiency of transformation was about 6.0%, it was easier to transform indica rice using the proposed method, and the transformation process was significantly shortened. The success of transformation was further confirmed by the genetic and molecular analyses of T (1) transformants.
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