Journal
PLANT CELL REPORTS
Volume 28, Issue 5, Pages 769-776Publisher
SPRINGER
DOI: 10.1007/s00299-009-0679-1
Keywords
Soybean; Transformation; Selectable marker; Rice; Acetolactate synthase; Herbicide resistance
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Funding
- Ministry of Agriculture, Forestry and Fisheries of Japan
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We investigated selective culturing conditions for the production of transgenic soybeans. In this culturing system, we used the acetolactate synthase (ALS)-inhibiting herbicide-resistance gene derived from rice (Os-mALS gene) as a selectable marker gene instead of that derived from bacteria, which interfered with the cultivation and practical usage of transgenic crops. T(1) soybeans grown from one regenerated plant after selection of the ALS-targeting pyrimidinyl carboxy (PC) herbicide bispyribac-sodium (BS) exhibited herbicide resistance, and the introduction and expression of the Os-mALS gene were confirmed by genetic analysis. The selective culturing system promoted by BS herbicide, in which the Os-mALS gene was used as a selectable marker, was proved to be applicable to the production of transgenic soybeans, despite the appearance of escaped soybean plants that did not contain the Os-mALS transgene.
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