Journal
PLANT CELL REPORTS
Volume 28, Issue 2, Pages 325-333Publisher
SPRINGER
DOI: 10.1007/s00299-008-0643-5
Keywords
In vitro culture; Growth regulator; Heavy metal tolerance; Antioxidant systems
Categories
Funding
- Knowledge Innovation Program of the Chinese Academy of Sciences [0707013603]
Ask authors/readers for more resources
Solanum nigrum is a newly discovered Cd-hyperaccumulator. In the present study, the protective effects of proline against cadmium toxicity of callus and regenerated shoots of S. nigrum are investigated based on a high frequency in vitro shoot regeneration system. Proline pretreatment reduces the reactive oxygen species levels and protects the plasma membrane integrity of callus under cadmium stress, and therefore improves the cadmium tolerance in S. nigrum. Inductively coupled plasma mass spectroscopy analysis shows that exogenous proline increases the cadmium accumulation in callus and regenerated shoots of S. nigrum. Further analysis indicates that the improvement of cadmium tolerance caused by proline pretreatment is correlated with an increase of superoxide dismutase and catalase activity and intracellular total glutathione content. The interaction between proline and enzymic or non-enzymic antioxidants is discussed.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available