Four Arabidopsis AREB/ABF transcription factors function predominantly in gene expression downstream of SnRK2 kinases in abscisic acid signalling in response to osmotic stress

Under osmotic stress conditions such as drought and high salinity, the plant hormone abscisic acid (ABA) plays important roles in stress-responsive gene expression mainly through three bZIP transcription factors, AREB1/ABF2, AREB2/ABF4 and ABF3, which are activated by SNF1-related kinase 2s (SnRK2s) such as SRK2D/SnRK2.2, SRK2E/SnRK2.6 and SRK2I/SnRK2.3 (SRK2D/E/I). However, since the three AREB/ABFs are crucial, but not exclusive, for the SnRK2-mediated gene expression, transcriptional pathways governed by SRK2D/E/I are not fully understood. Here, we show that a bZIP transcription factor, ABF1, is a functional homolog of AREB1, AREB2 and ABF3 in ABA-dependent gene expression in Arabidopsis. Despite lower expression levels of ABF1 than those of the three AREB/ABFs, the areb1 areb2 abf3 abf1 mutant plants displayed increased sensitivity to drought and decreased sensitivity to ABA in primary root growth compared with the areb1 areb2 abf3 mutant. Genome-wide transcriptome analyses revealed that expression of downstream genes of SRK2D/E/I, which include many genes functioning in osmotic stress responses and tolerance such as transcription factors and LEA proteins, was mostly impaired in the quadruple mutant. Thus, these results indicate that the four AREB/ABFs are the predominant transcription factors downstream of SRK2D/E/I in ABA signalling in response to osmotic stress during vegetative growth. Abscisic acid (ABA) plays important roles in osmotic stress-responsive gene expression mainly through three bZIP transcription factors, AREB1, AREB2, and ABF3, which are activated by SnRK2s such as SRK2D, SRK2E, and SRK2I (SRK2D/E/I). However, transcription factors other than the three AREB/ABFs that function downstream of SRK2D/E/I remain obscure. Here, we report that ABF1 is a functional homolog of AREB1, AREB2, and ABF3 in ABA-dependent gene expression from a comparative analysis between the areb1 areb2 abf3 abf1 and areb1 areb2 abf3 mutants. Moreover, genome-wide transcriptome analyses revealed that expression of downstream genes of SRK2D/E/I were mostly impaired in the areb1 areb2 abf3 abf1 quadruple mutant, suggesting that the four AREB/ABFs are the predominant transcription factors downstream of SRK2D/E/I in ABA signaling in response to osmotic stress.