Journal
PLANT CELL AND ENVIRONMENT
Volume 37, Issue 9, Pages 2036-2050Publisher
WILEY
DOI: 10.1111/pce.12275
Keywords
abscisic acid; brassinosteroids; hydrogen peroxide; NADPH oxidase; photosynthesis; stomata
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Funding
- National Basic Research Program of China [2009CB119000]
- National Natural Science Foundation of China [31000905]
- China Postdoctoral Science Foundation [20100481441, 201104742]
- Fundamental Research Funds for the Central Universities [2013FZA6012]
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Brassinosteroids (BRs) are essential for plant growth and development; however, their roles in the regulation of stomatal opening or closure remain obscure. Here, the mechanism underlying BR-induced stomatal movements is studied. The effects of 24-epibrassinolide (EBR) on the stomatal apertures of tomato (Solanum lycopersicum) were measured by light microscopy using epidermal strips of wild type (WT), the abscisic acid (ABA)-deficient notabilis (not) mutant, and plants silenced for SlBRI1, SlRBOH1 and SlGSH1. EBR induced stomatal opening within an appropriate range of concentrations, whereas high concentrations of EBR induced stomatal closure. EBR-induced stomatal movements were closely related to dynamic changes in H2O2 and redox status in guard cells. The stomata of SlRBOH1-silenced plants showed a significant loss of sensitivity to EBR. However, ABA deficiency abolished EBR-induced stomatal closure but did not affect EBR-induced stomatal opening. Silencing of SlGSH1, the critical gene involved in glutathione biosynthesis, disrupted glutathione redox homeostasis and abolished EBR-induced stomatal opening. The results suggest that transient H2O2 production is essential for poising the cellular redox status of glutathione, which plays an important role in BR-induced stomatal opening. However, a prolonged increase in H2O2 facilitated ABA signalling and stomatal closure.
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