Journal
PLANT CELL AND ENVIRONMENT
Volume 37, Issue 7, Pages 1672-1687Publisher
WILEY
DOI: 10.1111/pce.12280
Keywords
antioxidant enzymes; cadmium; dehydrogenases; NADPH; oxidative stress
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Funding
- European Regional Development Fund
- Ministerio de Economia y Competitividad [BIO2012-36742]
- Fundacion Ramon Areces, Spain
- European Social Fund (ESF)
- Consejo Superior de Investigaciones Cientificas
- Erasmus Mundus External Action 2
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Cadmium (Cd) is a non-essential heavy metal that may be toxic or even lethal to plants as it can be easily taken up by the roots and loaded into the xylem to the leaves. Using soybean roots (Glycine maxL.) DM 4800, we have analysed various parameters related to reactive oxygen metabolism and nitric oxide (NO) during a 6 day Cd exposure. A rise in H2O2 and NO, and to a lesser extent O-2(center dot-) content was observed after 6h exposure with a concomitant increase in lipid peroxidation and carbonyl group content. Both oxidative markers were significantly reduced after 24h. A second, higher wave of O-2(center dot-) production was also observed after 72h of exposure followed by a reduction until the end of the treatment. NOX and glicolate oxidase activity might be involved in the initial Cd-induced reactive oxygen species (ROS) production and it appears that other sources may also participate. The analysis of antioxidative enzymes showed an increase in glutathione-S-transferase activity and in transcript levels and activity of enzymes involved in the ascorbate-glutathione cycle and the NADPH-generating enzymes. These results suggest that soybean is able to respond rapidly to oxidative stress imposed by Cd by improving the availability of NADPH necessary for the ascorbate-glutathione cycle.
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