Journal
PLANT CELL AND ENVIRONMENT
Volume 37, Issue 10, Pages 2406-2420Publisher
WILEY
DOI: 10.1111/pce.12294
Keywords
laser microdissection; malic acid; metabolomics; microarray; rice; ROL barrier; VLCFA
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Funding
- Ministry of Agriculture, Forestry, and Fisheries of Japan [Genomics-based Technology for Agricultural Improvement] [GMO1005b]
- Japanese Society for Promotion of Science
- Grants-in-Aid for Scientific Research [11F01082, 12J05119] Funding Source: KAKEN
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The formation of a barrier to radial oxygen (O-2) loss (ROL) in the root is an important adaptation of plants to root flooding, but the biochemical changes in plant roots where the barrier is formed are unclear. In this study, we analysed metabolic profiles and gene expression profiles in roots of rice (Oryza sativaL.) plants grown under stagnant deoxygenated conditions, which induce suberization in the outer cell layers of the roots and formation of barrier to ROL. Under these conditions, two distinctive biochemical features of the roots were the accumulations of malic acid and very long chain fatty acids (VLCFAs). We also showed that the expressions of some genes encoding plastid-localized enzymes, which convert malic acid to acetyl coenzyme A (AcCoA), were simultaneously up-regulated under stagnant conditions. The expression levels of these genes in specific root tissues isolated by laser microdissection suggested that malic acid is converted to AcCoA predominantly in the plastids in the outer cell layers of rice roots. We propose that the physiological role of malic acid accumulation in rice roots grown under stagnant conditions is to provide a substrate for the biosynthesis of fatty acids, which, in turn, are used in the biosynthesis of suberin.
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