Journal
PLANT CELL AND ENVIRONMENT
Volume 36, Issue 11, Pages 2034-2045Publisher
WILEY
DOI: 10.1111/pce.12113
Keywords
chloroplasts; mitochondria; photosynthesis; redox homeostasis; reductive stress; ROS metabolism
Categories
Funding
- Ministry of Science and Higher Education (MNiSW, Poland) [N N303 401536]
- Faculty of Biology, University of Warsaw
Ask authors/readers for more resources
Ammonium nutrition has been suggested to be associated with alterations in the oxidation-reduction state of leaf cells. Herein, we show that ammonium nutrition in Arabidopsis thaliana increases leaf NAD(P)H/NAD(P)(+) ratio, reactive oxygen species content and accumulation of biomolecules oxidized by free radicals. We used the method of rapid fractionation of protoplasts to analyse which cellular compartments were over-reduced under ammonium supply and revealed that observed changes in NAD(P)H/NAD(P)(+) ratio involved only the extrachloroplastic fraction. We also showed that ammonium nutrition changes mitochondrial electron transport chain activity, increasing mitochondrial reactive oxygen species production. Our results indicate that the functional impairment associated with ammonium nutrition is mainly associated with redox reactions outside the chloroplast.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available