Journal
PLANT CELL AND ENVIRONMENT
Volume 36, Issue 1, Pages 186-199Publisher
WILEY
DOI: 10.1111/j.1365-3040.2012.02566.x
Keywords
phenotypic plasticity; transcriptome analysis; wood; xylogenesis
Categories
Funding
- Canada Research Chair Program
- Alberta Ingenuity New Faculty Award
- NSERC
- Genome British Columbia Applied Genomics Innovation Program
Ask authors/readers for more resources
Nitrogen availability has a strong influence on plant growth and development. In this study, we examined the effect of nitrogen availability on xylogenesis in hybrid poplar (Populus trichocarpa x deltoides H11-11). Saplings of hybrid poplar were fertilized for 33 d with either high or adequate levels of ammonium nitrate. We observed enhanced radial growth, wider vessels and fibres and thinner fibre walls in the secondary xylem of high N relative to adequate N plants. These anatomical differences translated into altered hydraulic properties with xylem being more transport efficient but also more vulnerable to drought-induced cavitation in high N plants. The changes in xylem structure and function were associated with differences in gene expression as revealed by the transcriptome analysis of the developing xylem region. We found 388 genes differentially expressed (fold change +/- 1.5, P-value <= 0.05), including a number of genes putatively involved in nitrogen and carbohydrate metabolism and various aspects of xylem cell differentiation. Several genes encoding known transcriptional regulators of secondary cell wall deposition were down-regulated in high N plants, corresponding with thinner secondary cell walls in these plants. The results of this study provide us with gene candidates potentially affecting xylem hydraulic and structural traits.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available