Journal
PLANT CELL AND ENVIRONMENT
Volume 36, Issue 5, Pages 1009-1018Publisher
WILEY
DOI: 10.1111/pce.12033
Keywords
Arabidopsis thaliana; GABA; glutamate; polyamines; salt tolerance; succinate; sugars; TCA cycle
Categories
Funding
- Ministere de l'Enseignement Superieur et de la Recherche
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Environmental constraints challenge cell homeostasis and thus require a tight regulation of metabolic activity. We have previously reported that the -aminobutyric acid (GABA) metabolism is crucial for Arabidopsis salt tolerance as revealed by the NaCl hypersensitivity of the GABA transaminase (GABA-T, At3g22200) gaba-t/pop2-1 mutant. In this study, we demonstrate that GABA-T deficiency during salt stress causes root and hypocotyl developmental defects and alterations of cell wall composition. A comparative genome-wide transcriptional analysis revealed that expression levels of genes involved in carbon metabolism, particularly sucrose and starch catabolism, were found to increase upon the loss of GABA-T function under salt stress conditions. Consistent with the altered mutant cell wall composition, a number of cell wall-related genes were also found differentially expressed. A targeted quantitative analysis of primary metabolites revealed that glutamate (GABA precursor) accumulated while succinate (the final product of GABA metabolism) significantly decreased in mutant roots after 1d of NaCl treatment. Furthermore, sugar concentration was twofold reduced in gaba-t/pop2-1 mutant roots compared with wild type. Together, our results provide strong evidence that GABA metabolism is a major route for succinate production in roots and identify GABA as a major player of central carbon adjustment during salt stress.
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