Journal
PLANT CELL AND ENVIRONMENT
Volume 33, Issue 12, Pages 2173-2179Publisher
WILEY
DOI: 10.1111/j.1365-3040.2010.02215.x
Keywords
amino acid synthesis; methyl jasmonate; plant defences; short-lived radiotracers
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Funding
- U.S. Department of Energy, Office of Biological and Environmental Research [DE-AC02-98CH10886]
- National Research Initiative of the USDA National Institute of Food and Agriculture [2007-35302-18351]
- Deutscher Akademischer Austauschdienst (DAAD), Bonn
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Nitrogen-13 (t(1/2) 9.97 m), a radioactive isotope of nitrogen, offers unique opportunities to explore plant nitrogen utilization over short time periods. Here we describe a method for administering 13N as gaseous 13NH(3) to intact leaves of Nicotiana tabacum L. (cv Samsun), and measuring the labelled amino acids using radio high-performance liquid chromatography (HPLC) on tissue extract. We used this method to study the effects of defence induction on plant nitrogen utilization by applying treatments of methyl jasmonate (MeJA), a potent defence elicitor. MeJA caused a significant increase relative to controls in key [13N]amino acids, including serine, glycine and alanine by 4 h post-treatment, yet had no effect on 13NH(3) incorporation, a process that is primarily under the control of the glutamine synthatase/glutamate synthase pathway (GS/GOGAT) in cellular photorespiration. We suggest that the reconfiguration of nitrogen metabolism may reflect induction of non-photorespiratory sources of nitrogen to better serve the plant's defences.
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