Journal
PLANT CELL
Volume 26, Issue 8, Pages 3272-3285Publisher
AMER SOC PLANT BIOLOGISTS
DOI: 10.1105/tpc.114.129460
Keywords
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Funding
- National Science Foundation [DBI-0604336, IOS-1025636]
- U.S. Department of Energy [DE-FG02-91ER20021]
- Michigan AgBioResearch Project [MICL02143]
- Division Of Integrative Organismal Systems
- Direct For Biological Sciences [1025636] Funding Source: National Science Foundation
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Flavonoids are ubiquitous plant aromatic specialized metabolites found in a variety of cell types and organs. Methylated flavonoids are detected in secreting glandular trichomes of various Solanum species, including the cultivated tomato (Solanum lycopersicum). Inspection of the sequenced S. lycopersicum Heinz 1706 reference genome revealed a close homolog of Solanum habrochaites MOMT1 3'/5' myricetin O-methyltransferase gene, but this gene (Solyc06g083450) is missing the first exon, raising the question of whether cultivated tomato has a distinct 3' or 3'/5' O-methyltransferase. A combination of mining genome and cDNA sequences from wild tomato species and S. lycopersicum cultivar M82 led to the identification of SI-MOMT4 as a 3' O-methyltransferase. In parallel, three independent ethyl methanesulfonate mutants in the S. lycopersicum cultivar M82 background were identified as having reduced amounts of di-and trimethylated myricetins and increased monomethylated myricetin. Consistent with the hypothesis that SI-MOMT4 is a 3' O-methyltransferase gene, all three myricetin methylation defective mutants were found to have defects in MOMT4 sequence, transcript accumulation, or 3'-O-methyltransferase enzyme activity. Surprisingly, no MOMT4 sequence is found in the Heinz 1706 reference genome sequence, and this cultivar accumulates 3-methyl myricetin and is deficient in 3'-methyl myricetins, demonstrating variation in this gene among cultivated tomato varieties.
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