Journal
PLANT CELL
Volume 26, Issue 3, Pages 1308-1329Publisher
OXFORD UNIV PRESS INC
DOI: 10.1105/tpc.113.122226
Keywords
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Funding
- European Union [HPRN-CT-2002-00262, LSH-2002-1.2.5-2]
- Biotechnology and Biological Sciences Research Council [BB/D016223/1]
- Leverhulme Trust [F/10 105/E]
- University of Leeds
- Brazilian National Council for Scientific and Technological Development
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo [2009/50650-6]
- Biotechnology and Biological Sciences Research Council [BB/K007181/1, BB/D016223/1] Funding Source: researchfish
- BBSRC [BB/K007181/1, BB/D016223/1] Funding Source: UKRI
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The cycling of vacuolar sorting receptors (VSRs) between early and late secretory pathway compartments is regulated by signals in the cytosolic tail, but the exact pathway is controversial. Here, we show that receptor targeting in tobacco (Nicotiana tabacum) initially involves a canonical coat protein complex II-dependent endoplasmic reticulum-to-Golgi bulk flow route and that VSR-ligand interactions in the cis-Golgi play an important role in vacuolar sorting. We also show that a conserved Glu is required but not sufficient for rate-limiting YXX phi-mediated receptor trafficking. Protein-protein interaction studies show that the VSR tail interacts with the mu-subunits of plant or mammalian clathrin adaptor complex AP1 and plant AP4 but not that of plant and mammalian AP2. Mutants causing a detour of full-length receptors via the cell surface invariantly cause the secretion of VSR ligands. Therefore, we propose that cycling via the plasma membrane is unlikely to play a role in biosynthetic vacuolar sorting under normal physiological conditions and that the conserved Ile-Met motif is mainly used to recover mistargeted receptors. This occurs via a fundamentally different pathway from the prevacuolar compartment that does not mediate recycling. The role of clathrin and clathrin-independent pathways in vacuolar targeting is discussed.
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