Journal
PLANT CELL
Volume 25, Issue 8, Pages 3104-3116Publisher
OXFORD UNIV PRESS INC
DOI: 10.1105/tpc.113.116343
Keywords
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Funding
- UK Biotechnology and Biological Sciences Research Council through a studentship at the University of Warwick
- UK Biotechnology and Biological Sciences Research Council through an Institute Strategic Program grant at Rothamsted Research
- Biotechnology and Biological Sciences Research Council [BB/E022197/1, BBS/E/H/00PE0274] Funding Source: researchfish
- BBSRC [BB/E022197/1, BBS/E/H/00PE0274] Funding Source: UKRI
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Arabidopsis thaliana seed maturation is accompanied by the deposition of storage oil, rich in the essential omega-3 polyunsaturated fatty acid alpha-linolenic acid (ALA). The synthesis of ALA is highly responsive to the level of FATTY ACID DESATURASE3 (FAD3) expression, which is strongly upregulated during embryogenesis. By screening mutants in LEAFY COTYLEDON1 (LEC1)-inducible transcription factors using fatty acid profiling, we identified two mutants (lec1-like and bzip67) with a seed lipid phenotype. Both mutants share a substantial reduction in seed ALA content. Using a combination of in vivo and in vitro assays, we show that bZIP67 binds G-boxes in the FAD3 promoter and enhances FAD3 expression but that activation is conditional on bZIP67 association with LEC1- LIKE (L1L) and NUCLEAR FACTOR-YC2 (NF-YC2). Although FUSCA3 and ABSCISIC ACID INSENSITIVE3 are required for L1L and bZIP67 expression, neither protein is necessary for [bZIP67:L1L:NF-YC2] to activate FAD3. We conclude that a transcriptional complex containing L1L, NF-YC2, and bZIP67 is induced by LEC1 during embryogenesis and specifies high levels of ALA production for storage oil by activating FAD3 expression.
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