Journal
PLANT CELL
Volume 25, Issue 6, Pages 2056-2069Publisher
OXFORD UNIV PRESS INC
DOI: 10.1105/tpc.113.111922
Keywords
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Funding
- RIKEN Biomass Engineering Program
- RIKEN Plant Science Center
- Japan Society for the Promotion of Science [18870028, 24770052, 18370016, 22370015]
- Ministry of Education, Culture, Sports, Science, and Technology of Japan [19060001]
- Grants-in-Aid for Scientific Research [18370016, 24770052, 22370015, 24114002, 19060001, 18870028, 24114001, 25114520] Funding Source: KAKEN
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Pre-mRNA splicing is a critical process in gene expression in eukaryotic cells. A multitude of proteins are known to be involved in pre-mRNA splicing in plants; however, the physiological roles of only some of these have been examined. Here, we investigated the developmental roles of a pre-mRNA splicing factor by analyzing root initiation defective1-1 (rid1-1), an Arabidopsis thaliana mutant previously shown to have severe defects in hypocotyl dedifferentiation and de novo meristem formation in tissue culture under high-temperature conditions. Phenotypic analysis in planta indicated that RID1 is differentially required during development and has roles in processes such as meristem maintenance, leaf morphogenesis, and root morphogenesis. RID1 was identified as encoding a DEAH-box RNA helicase implicated in pre-mRNA splicing. Transient expression analysis using intron-containing reporter genes showed that pre-mRNA splicing efficiency was affected by the rid1 mutation, which supported the presumed function of RID1 in pre-mRNA splicing. Our results collectively suggest that robust levels of pre-mRNA splicing are critical for several specific aspects of plant development.
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