Journal
PLANT CELL
Volume 24, Issue 8, Pages 3393-3405Publisher
AMER SOC PLANT BIOLOGISTS
DOI: 10.1105/tpc.112.100933
Keywords
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Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan [22119004, 07088014, 21780314]
- Science and Technology Research Partnership for Sustainable Development of the Japan Science and Technology Agency/Japan International Cooperation Agency
- Genomics for Agricultural Innovation
- Development of Abiotic Stress-Tolerant Crops by DREB Genes from the Ministry of Agriculture, Forestry, and Fisheries of Japan
- Program for the Promotion of Basic Research Activities for Innovative Biosciences of Japan
- Grants-in-Aid for Scientific Research [21780314, 22119004] Funding Source: KAKEN
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Arabidopsis thaliana DEHYDRATION-RESPONSIVE ELEMENT BINDING PROTEIN2A (DREB2A) functions as a transcriptional activator that increases tolerance to osmotic and heat stresses; however, its expression also leads to growth retardation and reduced reproduction. To avoid these adverse effects, the expression of DREB2A is predicted to be tightly regulated. We identified a short promoter region of DREB2A that represses its expression under nonstress conditions. Yeast one-hybrid screening for interacting factors identified GROWTH-REGULATING FACTOR7 (GRF7). GRF7 bound to the DREB2A promoter and repressed its expression. In both artificial miRNA-silenced lines and a T-DNA insertion line of GRF7, DREB2A transcription was increased compared with the wild type under nonstress conditions. A previously undiscovered cis-element, GRF7-targeting cis-element (TGTCAGG), was identified as a target sequence of GRF7 in the short promoter region of DREB2A via electrophoretic mobility shift assays. Microarray analysis of GRF7 knockout plants showed that a large number of the upregulated genes in the mutant plants were also responsive to osmotic stress and/or abscisic acid. These results suggest that GRF7 functions as a repressor of a broad range of osmotic stress-responsive genes to prevent growth inhibition under normal conditions.
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