Journal
PLANT CELL
Volume 24, Issue 2, Pages 551-565Publisher
AMER SOC PLANT BIOLOGISTS
DOI: 10.1105/tpc.111.094284
Keywords
-
Funding
- National Basic Research Program of China (973 Program) [2011CB809103, 2011CB944601]
- Chinese Academy of Sciences/State Administration of Foreign Expert Affairs International Partnership Program for Creative Research Teams [20090491019]
- National Natural Science Foundation of China [31000595, 30730009, 30821007]
- Chinese Academy of Sciences [KJCX2YW-L08, KSCX2-EW-J-1]
- China Postdoctoral Science Foundation
- Agency Vedecka Grantova Agentura (Bratislava, Slovakia) [2/0200/10]
- Agency Agentura na Podporu Vyskumu a Vyvoja (Bratislava, Slovakia) [APVV-0432-06]
- Bundesministeriums fur Wirtschaft und Technologie via Deutsches Zentrum fur Luft-und Raumfahrt (Cologne, Germany) [50WB 0434]
- European Space Agency-European Space Research and Technology Centre (Noordwijk, The Netherlands) [AO-99098]
- Ente Cassa di Risparmio di Firenze (Italy)
Ask authors/readers for more resources
Under blue light (BL) illumination, Arabidopsis thaliana roots grow away from the light source, showing a negative phototropic response. However, the mechanism of root phototropism is still unclear. Using a noninvasive microelectrode system, we showed that the BL sensor phototropin1 (phot1), the signal transducer NONPHOTOTROPIC HYPOCOTYL3 (NPH3), and the auxin efflux transporter PIN2 were essential for BL-induced auxin flux in the root apex transition zone. We also found that PIN2-green fluorescent protein (GFP) localized to vacuole-like compartments (VLCs) in dark-grown root epidermal and cortical cells, and phot1/NPH3 mediated a BL-initiated pathway that caused PIN2 redistribution to the plasma membrane. When dark-grown roots were exposed to brefeldin A (BFA), PIN2-GFP remained in VLCs in darkness, and BL caused PIN2-GFP disappearance from VLCs and induced PIN2-GFP-FM4-64 colocalization within enlarged compartments. In the nph3 mutant, both dark and BL BFA treatments caused the disappearance of PIN2-GFP from VLCs. However, in the phot1 mutant, PIN2-GFP remained within VLCs under both dark and BL BFA treatments, suggesting that phot1 and NPH3 play different roles in PIN2 localization. In conclusion, BL-induced root phototropism is based on the phot1/NPH3 signaling pathway, which stimulates the shootward auxin flux by modifying the subcellular targeting of PIN2 in the root apex transition zone.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available