Journal
PLANT CELL
Volume 24, Issue 10, Pages 4135-4148Publisher
AMER SOC PLANT BIOLOGISTS
DOI: 10.1105/tpc.112.102608
Keywords
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Funding
- Chinese National Scientific Foundation [31070163]
- Chinese Academy of Sciences [KSCX2-EW-Q-1-04]
- National Basic Research Program of China (973 Program) [2012CB910503]
- Marie Curie Intra-European Fellowship (EU FP7)
- University Research Fellowship from the Royal Society
- BBSRC [BBS/E/J/000C0657] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/E/J/000C0657] Funding Source: researchfish
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Eukaryotic organisms have evolved a set of strategies to safeguard genome integrity, but the underlying mechanisms remain poorly understood. Here, we report that ASYMMETRIC LEAVES1/2 ENHANCER7 (AE7), an Arabidopsis thaliana gene encoding a protein in the evolutionarily conserved Domain of Unknown Function 59 family, participates in the cytosolic iron-sulfur (Fe-S) cluster assembly (CIA) pathway to maintain genome integrity. The severe ae7-2 allele is embryo lethal, whereas plants with the weak ae7 (ae7-1) allele are viable but exhibit highly accumulated DNA damage that activates the DNA damage response to arrest the cell cycle. AE7 is part of a protein complex with CIA1, NAR1, and MET18, which are highly conserved in eukaryotes and are involved in the biogenesis of cytosolic and nuclear Fe-S proteins. ae7-1 plants have lower activities of the cytosolic [4Fe-4S] enzyme aconitase and the nuclear [4Fe-4S] enzyme DNA glycosylase ROS1. Additionally, mutations in the gene encoding the mitochondrial ATP binding cassette transporter ATM3/ABCB25, which is required for the activity of cytosolic Fe-S enzymes in Arabidopsis, also result in defective genome integrity similar to that of ae7-1. These results indicate that AE7 is a central member of the CIA pathway, linking plant mitochondria to nuclear genome integrity through assembly of Fe-S proteins.
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