Journal
PLANT CELL
Volume 23, Issue 5, Pages 1701-1705Publisher
AMER SOC PLANT BIOLOGISTS
DOI: 10.1105/tpc.110.082016
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Funding
- Deutsches Zentrum fur Luft- und Raumfahrt e.V.
- Landesstiftung BW
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Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) is a widespread method for metabolic labeling of cells and tissues in quantitative proteomics; however, incomplete incorporation of the label has so far restricted its wider use in plants. Here, we argue that differential labeling by two different versions of the labeled amino acids renders SILAC fully applicable to dark-grown plant cell lines. By comparing Arabidopsis thaliana cell cultures labeled with two versions of heavy Lys (Lys-4 and Lys-8), we show that this simple modification of the SILAC protocol enables similar quantitation accuracy, precision, and reproducibility as conventional SILAC in animal cells.
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