Journal
PLANT BREEDING
Volume 129, Issue 2, Pages 176-180Publisher
WILEY-BLACKWELL PUBLISHING, INC
DOI: 10.1111/j.1439-0523.2009.01669.x
Keywords
blast disease resistance; flanking markers; marker-assisted selection; pyramiding; tightly-linked markers; stepwise screening
Funding
- Rockefeller Foundation
- National Center for Genetic Engineering and Biotechnology (BIOTEC)
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P>The rice varieties IR64 and Jao Hom Nin (JHN) demonstrated a broad-spectrum resistance against the rice blast pathogens in Thailand. A genomic investigation unravelled many resistance genes residing on four genomic regions, chromosome 2 and 12 in IR64 and 1 and 11 in JHN. A cross between these varieties was made to combine resistance genes into a single genotype. Marker-assisted selection (MAS) was employed to identify F(2) and F(3) plants carrying a combination of four resistance QTLs in a homozygous fusion. Flanking markers RM212/RM319 and RM144/RM139 to blast resistant QTLs on chromosome 1 and 11 in JHN rice and tightly-linked markers RM208 and RM179 to blast resistant QTLs on chromosome 2 and 12 in IR64 rice variety were used for MAS. The stepwise MAS screening was brought in as a strategy to provide a cost-saving and minimum number of PCR performing to select resistant genotypes. F(4) generation, lines carrying all resistant QTLs show a broader spectrum of resistance against 11 representatives of Thai blast pathogen isolates.
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