Journal
PLANT BIOTECHNOLOGY JOURNAL
Volume 17, Issue 2, Pages 499-504Publisher
WILEY
DOI: 10.1111/pbi.12993
Keywords
base editing; adenine base editor; cytosine base editor; Cas9 variants; rice
Funding
- Chinese Academy of Sciences
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Base editing is a novel genome editing strategy that enables irreversible base conversion at target loci without the need for double stranded break induction or homology-directed repair. Here, we developed new adenine and cytosine base editors with engineered SpCas9 and SaCas9 variants that substantially expand the targetable sites in the rice genome. These new base editors can edit endogenous genes in the rice genome with various efficiencies. Moreover, we show that adenine and cytosine base editing can be simultaneously executed in rice. The new base editors described here will be useful in rice functional genomics research and will advance precision molecular breeding in crops.
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