Journal
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
Volume 36, Issue 1, Pages 19-24Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/ATVBAHA.115.306681
Keywords
choroidal neovascularization; diabetic retinopathy; macular degeneration; myeloid cells; vascular endothelial growth factor A
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Funding
- joint Medical Research Council
- Fight for Sight grant [MR/K003003/1]
- People Programme (Marie Curie Actions)
- European Union's Seventh Framework Programme (FP7) under Research Executive Agency [629556]
- Special Trustees of Moorfields Eye Hospital [ST 1503B]
- Wellcome Trust [095623/Z/11/Z]
- Medical Research Council [MR/K003003/1, MR/L022699/1] Funding Source: researchfish
- National Institute for Health Research [NF-SI-0513-10074, NIHR-RP-011-003] Funding Source: researchfish
- Fight for Sight [1333/34] Funding Source: researchfish
- MRC [MR/L022699/1, MR/K003003/1] Funding Source: UKRI
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Objective Ocular neovascularization (ONV) is a pathological feature of sight-threatening human diseases, such as diabetic retinopathy and age-related macular degeneration. Macrophage depletion in mouse models of ONV reduces the formation of pathological blood vessels, and myeloid cells are widely considered an important source of the vascular endothelial growth factor A (VEGF). However, the importance of VEGF or its upstream regulators hypoxia-inducible factor-1 (HIF1) and hypoxia-inducible factor-2 (HIF2) as myeloid-derived regulators of ONV remains to be determined. Approach and Results We used 2 mouse models of ONV, choroidal neovascularization and oxygen-induced retinopathy, to show that Vegfa is highly expressed by several cell types, but not myeloid cells during ONV. Moreover, myeloid-specific VEGF ablation did not reduce total ocular VEGF during choroidal neovascularization or oxygen-induced retinopathy. In agreement, the conditional inactivation of Vegfa, Hif1a, or Epas1 in recruited and resident myeloid cells that accumulated at sites of neovascularization did not significantly reduce choroidal neovascularization or oxygen-induced retinopathy. Conclusions The finding that myeloid cells are not a significant local source of VEGF in these rodent models of ONV suggests that myeloid function in neovascular eye disease differs from skin wound healing and other neovascular pathologies.
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