Journal
PLANT BIOTECHNOLOGY
Volume 29, Issue 4, Pages 419-423Publisher
JAPANESE SOC PLANT CELL & MOLECULAR BIOLOGY
DOI: 10.5511/plantbiotechnology.12.0627a
Keywords
High-throughput; lignin; microscale; thioacidolysis; beta-O-4 substructure
Funding
- Research and development of genetic and metabolic information of energy producing plants for breeding programs from the New Energy and Industrial Technology Development Organization (NEDO)
- Japan Society for Promotion of Science [20380102, 23658144]
- Grants-in-Aid for Scientific Research [23658144, 20380102] Funding Source: KAKEN
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Thioacidolysis is a method to detect the beta-O-4 substructures of lignin, and has been employed as a diagnostic test for the presence of lignin. However, the conventional thioacidolysis protocol is low-throughput and is a bottleneck in the characterization of lignins in a large number of samples such as transgenic lines. Recently, a rapid analysis protocol for thioacidolysis was reported. In this study, we modified mainly the work-up process. Our microscale protocol showed higher yields of thioacidolysis products than the conventional protocol.
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