Journal
PLANT BIOTECHNOLOGY
Volume 26, Issue 3, Pages 275-284Publisher
JAPANESE SOC PLANT CELL & MOLECULAR BIOLOGY
DOI: 10.5511/plantbiotechnology.26.275
Keywords
Binary vector; epitope tag; Gateway cloning; reporter
Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan [17027020, 19039025, 17027011, 17051013, 19039014, 19043008]
- Japan Society for Promotion of Science [17510066, 16370020]
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The availability of complete plant genome sequences has opened a new era of plant molecular research using approaches such as comprehensive expression analysis, ectopic expression and gene knockdown. For Such purposes, new tools realizing easy and rapid construction of recombinant plasmids are required. Here, we describe Gateway cloning compatible binary vectors for post-genomic research in plant genetic engineering. The vectors comprise a variety of reporters, epitope tags and selective markers that should make them useful for construction of plasmids for Agrobacterium-mediated transformation of plants. We also describe vectors for promoter swapping using Gateway cloning technology.
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