Journal
PLANT AND SOIL
Volume 385, Issue 1-2, Pages 287-302Publisher
SPRINGER
DOI: 10.1007/s11104-014-2199-1
Keywords
N-15 techniques; Isotope dilution; Natural abundance; Fertiliser nitrogen; White clover; N fixation
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Funding
- EU
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Aims The objectives of this study were to compare techniques for measuring biological nitrogen fixation (BNF) and to assess how fertiliser N input affects the balance between BNF and sustainable herbage production on perennial ryegrass (Lolium perenne L.)/white clover (Trifolium repens L.) grassland. Methods Biological N fixation and herbage production by white clover based grassland was measured in 2011 and 2012 under four nominal annual fertiliser N inputs: 0 (0N), 86 (86N), 140 (140N) and 280 kg ha(-1) (280N). Biological N fixation was measured using the N-15 isotope dilution and N-15 natural abundance techniques under all fertiliser N inputs and also using the nitrogen difference technique under 0N. Results The two N-15 techniques produced similar annual estimates of above-ground BNF across the range of fertiliser N inputs. Fertiliser N input resulted in higher herbage dry matter yield, but reduced annual BNF which averaged 80, 64, 66 and 47 kg ha(-1) on 0N, 86N, 140N and 280N, respectively, across both techniques and years. Conclusions The two N-15 techniques were comparable in estimating BNF. Intermediate fertiliser N inputs achieved a balance between minimising detrimental impact on BNF and potential N loss while producing more herbage DM yield than clover swards receiving no fertiliser N.
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