Journal
PLANT AND SOIL
Volume 345, Issue 1-2, Pages 387-400Publisher
SPRINGER
DOI: 10.1007/s11104-011-0793-z
Keywords
Nitrate reductase; SPAD; Ammonium transporter; Petiole nitrate concentration; Solanum tuberosum
Categories
Funding
- New Brunswick Department of Agriculture and Aquaculture
- Agriculture and Agri-Food Canada
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Use of an in-season measure of crop N sufficiency to guide fertilizer management is one approach to match the supply of N to the crop N demand. This study examined use of gene expression in leaf tissue of field-grown potatoes for use in assessment of potato N sufficiency. Potato cultivar 'Shepody' was grown with six fertilizer N rates (0-250 kg N ha(-1)). Leaf disks were collected weekly for quantification of the expression of N uptake/transport, N assimilation, and amino acid metabolism genes in leaf tissue by nCounter. Many of the genes evaluated were responsive to crop N supply, but the response varied widely among sampling dates. The exception was an ammonium transporter gene (AT1) which was highly expressed, was relatively consistent across sampling dates, was closely related to root zone soil nitrate concentration across N rates and sampling dates, and was highly negatively correlated with total tuber yield. The level of expression of AT1 in leaf tissue was as good as or better than conventional chemical or optical measures of potato N sufficiency in the current study.
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