Journal
PLANT AND CELL PHYSIOLOGY
Volume 55, Issue 6, Pages 1096-1109Publisher
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcu045
Keywords
GR24; High-tillering dwarf; SCF complex; Shoot branching; Strigolactones
Categories
Funding
- '973' project [2013CBA01401]
- National Natural Science Foundation of China [31271311]
- Agricultural Science and Technology Innovation Program of China
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Strigolactones (SLs) are a novel class of plant hormones that inhibit shoot branching. Currently, two proteins in rice are thought to play crucial roles in SL signal transduction. DWARF14 (D14), an alpha/beta hydrolase, is responsible for SL perception, while DWARF3 (D3), an F-box protein with leucine-rich repeats, is essential for SL signal transduction. However, how these two proteins transmit SL signals to downstream factors remains unclear. Here, we characterized a high-tillering dwarf rice mutant, gsor300097, which is insensitive to GR24, a synthetic analog of SL. Mapping and sequencing analysis showed that gsor300097 is a novel allelic mutant of D3, in which a nonsense mutation truncates the protein from 720 to 527 amino acids. The D3 gene was strongly expressed in root, leaf, shoot base and panicle. Nuclear-localized F-box protein D3 played a role in the SCF complex by interacting with OSK1, OSK5 or OSK20 and OsCullin1. In addition, D3 associated with D14 in a GR24-dependent manner in vivo. Taken together, our findings suggested that D3 assembled into an SCFD3 complex and associated with D14 to suppress rice shoot branching.
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