4.7 Article

Differential Transcriptomic Analysis by RNA-Seq of GSNO-Responsive Genes Between Arabidopsis Roots and Leaves

Journal

PLANT AND CELL PHYSIOLOGY
Volume 55, Issue 6, Pages 1080-1095

Publisher

OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcu044

Keywords

Arabidopsis; Massively parallel sequencing; Methionine sulfoxide reductase; Nitric oxide; Plant signaling mechanisms; RNA-seq; S-nitrosoglutathione

Funding

  1. Ministry of Science and Innovation [BIO2009-12003-C02-01, BIO2009-12003-C02-02, BIO2012-33904]
  2. Junta de Andalucia, Spain [group BIO286, BIO192]
  3. Ministry of Science and Innovation

Ask authors/readers for more resources

S-Nitrosoglutathione (GSNO) is a nitric oxide-derived molecule that can regulate protein function by a post-translational modification designated S-nitrosylation. GSNO has also been detected in different plant organs under physiological and stress conditions, and it can also modulate gene expression. Thirty-day-old Arabidopsis plants were grown under hydroponic conditions, and exogenous 1 mM GSNO was applied to the root systems for 3 h. Differential gene expression analyses were carried out both in roots and in leaves by RNA sequencing (RNA-seq). A total of 3,263 genes were identified as being modulated by GSNO. Most of the genes identified were associated with the mechanism of protection against stress situations, many of these having previously been identified as target genes of GSNO by array-based methods. However, new genes were identified, such as that for methionine sulfoxide reductase (MSR) in leaves or different miscellaneous RNA (miscRNA) genes in Arabidopsis roots. As a result, 1,945 GSNO-responsive genes expressed differently in leaves and roots were identified, and 114 of these corresponded exclusively to one of these organs. In summary, it is demonstrated that RNA-seq extends our knowledge of GSNO as a signaling molecule which differentially modulates gene expression in roots and leaves under non-stress conditions.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available