Journal
PLANT AND CELL PHYSIOLOGY
Volume 54, Issue 12, Pages 1963-1975Publisher
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pct135
Keywords
Aquaporin; Circadian regulation; Leaf hydraulic conductance (K-leaf); Light; Poplar (Populus trichocarpa; Populus nigra); Promoter
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Funding
- Ministere de la recherche et de l'enseignement superieur
- Interuniversity Attraction Poles Programme-Belgian Science Policy
- Communaute francaise de Belgique-Actions de Recherches Concertees
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To help understand leaf hydraulic conductance (K-leaf) modulation under high irradiance, well-watered poplars (Populus trichocarpa Torr. & Gray ex Hook and Populus nigra L.) were studied diurnally at molecular and ecophysiological scales. Transcriptional and translational modulations of plasma membrane intrinsic protein (PIP) aquaporins were evaluated in leaf samples during diurnal time courses. Among the 15 poplar PIP genes, a subset of two PIP1s and seven PIP2s are precociously induced within the first hour of the photoperiod concomitantly with a K-leaf increase. Since expression patterns were cyclic and reproducible over several days, we hypothesized that endogenous signals could be involved in PIP transcriptional regulation. To address this question, plants were submitted to forced darkness during their subjective photoperiod and compared with their control counterparts, which showed that some PIP1s and PIP2s have circadian regulation while others did not. Promoter analysis revealed that a large number of hormone, light, stress response and circadian elements are present. Finally, involvement of aquaporins is supported by the reduction of K-leaf by HgCl2 treatment.
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