Journal
PLANT AND CELL PHYSIOLOGY
Volume 53, Issue 3, Pages 518-527Publisher
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcs007
Keywords
Acaryochloris marina; Chlorophyllide a oxygenase; Metabolic engineering; Novel Chl; Transformation
Categories
Funding
- The Japan Society for the Promotion of Science [17GS0314]
- Ministry of Education, Culture, Sports, Science and Technology of Japan [21570038, 22750017, 22245030, 22370017]
- Japan Science and Technology Agency
- Grants-in-Aid for Scientific Research [22370017, 21570038, 22750017, 24658080, 22245030] Funding Source: KAKEN
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In oxygenic photosynthetic organisms, the properties of photosynthetic reaction systems primarily depend on the Chl species used. Acquisition of new Chl species with unique optical properties may have enabled photosynthetic organisms to adapt to various light environments. The artificial production of a new Chl species in an existing photosynthetic organism by metabolic engineering provides a model system to investigate how an organism responds to a newly acquired pigment. In the current study, we established a transformation system for a Chl d-dominated cyanobacterium, Acaryochloris marina, for the first time. The expression vector (constructed from a broad-host-range plasmid) was introduced into A. marina by conjugal gene transfer. The introduction of a gene for chlorophyllide a oxygenase, which is responsible for Chl b biosynthesis, into A. marina resulted in a transformant that synthesized a novel Chl species instead of Chl b. The content of the novel Chl in the transformant was approximately 10% of the total Chl, but the level of Chl a, another Chl in A. marina, did not change. The chemical structure of the novel Chl was determined to be [7-formyl]-Chl d(P) by mass spectrometry and nuclear magnetic resonance spectroscopy. [7-Formyl]-Chl d(P) is hypothesized to be produced by the combined action of chlorophyllide a oxygenase and enzyme(s) involved in Chl d biosynthesis. These results demonstrate the flexibility of the Chl biosynthetic pathway for the production of novel Chl species, indicating that a new organism with a novel Chl might be discovered in the future.
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