Journal
PLANT AND CELL PHYSIOLOGY
Volume 50, Issue 7, Pages 1292-1304Publisher
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcp073
Keywords
Affinity chromatography; Arabidopsis thaliana; Boron; Membrane; Proteomics; Zea mays
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Funding
- Deutsche Forschungsgemeinschaft [WI 2685/1-1, WI 2685/1-2]
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Boron deficiency symptoms point to a role for boron in plant membranes, but the molecular partners interacting with boron have not yet been identified. The objective of the present study was to isolate and identify membrane-associated proteins with an ability to interact with boron. Boron-interacting proteins were isolated from root microsomal preparations of arabidopsis (Arabidopsis thaliana) and maize (Zea mays) using phenylboronate affinity chromatography, subsequently separated by two-dimensional gel electrophoresis and identified using MALDI-TOF (matrix-assisted laser desorption ionization-time of flight) peptide mass fingerprinting. Twenty-six boron-binding membrane-associated proteins were identified in A. thaliana, and nine in Z. mays roots. Additional uniden-tified proteins were also present. Common to both species were the -subunit of mitochondrial ATP synthase, several -glucosidases, a luminal-binding protein and fructose bisphosphate aldolase. In A. thaliana, binding of these proteins to boron was significantly reduced after 4 d of boron deprivation. The relatively high number of diverse proteins identified as boron interacting, many of which are usually enriched in membrane microdomains, supports the hypothesis that boron plays a role in plant membranes by cross-linking glycoproteins, and may be involved in their recruitment to membrane microdomains.
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