Journal
PLANT AND CELL PHYSIOLOGY
Volume 50, Issue 7, Pages 1387-1392Publisher
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcp071
Keywords
Gene silencing; Glutelin promoter; Myo-inositol 3-phosphate synthase; Oryza sativa L; Phytic acid; Transgenic plants
Categories
Funding
- Plants, Insects and Animals Using Genome Technology [IP-5007]
- Ministry of Agriculture, Forestry, and Fisheries of Japan [15380004]
- Grants-in-Aid for Scientific Research [15380004] Funding Source: KAKEN
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In previous studies, we attempted to reduce phytic acid in rice seeds by silencing the 1d-myo-inositol 3-phosphate synthase gene, RINO1, using an antisense sequence under the control of the rice glutelin GluB-1 promoter. The stable transgenic line showed a weak low phytic acid phenotype. In this study, we show that the position of the caryopsis in the panicle might affect the level of gene silencing through a difference in temporal and spatial expression patterns between RINO1 and GluB-1 promoters, resulting in a large variation in Pi levels and a small increase in Pi in the transgenic seeds.
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