Journal
PLANT AND CELL PHYSIOLOGY
Volume 50, Issue 5, Pages 1012-1016Publisher
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcp053
Keywords
Betalain; Betalamic acid; DOPA dioxygenase; Mirabilis jalapa
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Betalains are synthesized in flowers, fruits and other tissues of the plant order Caryophyllales. Betalamic acid is the chromophore of betalain pigments synthesized by a ring-cleaving enzyme reaction on l-dihydroxyphenylalanine (DOPA). Although reverse genetic evidence has proven that DOPA 4,5-dioxygenase (DOD) is a key enzyme of betalain biosynthesis, all attempts to detect recombinant plant DOD activity in vitro have failed. Here, we report on the formation of betalamic acid from DOPA under suitable assay conditions using recombinant MjDOD produced by Escherichia coli. This is the first report showing biochemical evidence for DOD activity in vitro.
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