Journal
PHYTOTHERAPY RESEARCH
Volume 29, Issue 1, Pages 67-72Publisher
WILEY-BLACKWELL
DOI: 10.1002/ptr.5227
Keywords
patchouli alcohol; Helicobacter pylori; urease; antibacterial; non-competitive inhibitor
Categories
Funding
- Guangdong International Cooperation Project [2012B050300002]
- National Science Foundation of China [81374043, 81173534]
- Science and Technological Program for Dongguan's Higher Education, Science and Research, and Health Care Institutions [2012105102009]
- PhD Programs Foundation of Ministry of Education of China [20134425110009]
- Science and technology innovation project of Guangdong Provincial Department of Education [2013KJCX0045]
Ask authors/readers for more resources
The aim of this study is to evaluate the antibacterial activity and urease inhibitory effects of patchouli alcohol (PA), the bioactive ingredient isolated from Pogostemonis Herba, which has been widely used for the treatment of gastrointestinal disorders. The activities of PA against selected bacteria and fungi were determined by agar dilution method. It was demonstrated that PA exhibited selective antibacterial activity against Helicobacter pylori, without influencing the major normal gastrointestinal bacteria. Noticeably, the antibacterial activity of PA was superior to that of amoxicillin, with minimal inhibition concentration value of 78 mu g/mL. On the other hand, PA inhibited ureases from H.pylori and jack bean in concentration-dependent fashion with IC50 values of 2.67 +/- 0.79mM and 2.99 +/- 0.41mM, respectively. Lineweaver-Burk plots indicated that the type of inhibition was non-competitive against H.pylori urease whereas uncompetitive against jack bean urease. Reactivation of PA-inactivated urease assay showed DL-dithiothreitol, the thiol reagent, synergistically inactivated urease with PA instead of enzymatic activity recovery. In conclusion, the selective H.pylori antibacterial activity along with urease inhibitory potential of PA could make it a possible drug candidate for the treatment of H.pylori infection. Copyright (c) 2014 John Wiley & Sons, Ltd.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available