Journal
PHYTOTHERAPY RESEARCH
Volume 26, Issue 1, Pages 106-112Publisher
WILEY
DOI: 10.1002/ptr.3510
Keywords
antioxidant; blackberries; skin; ultraviolet; anthocyanin
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Funding
- Kentucky Tobacco Research and Development Center at the University of Kentucky
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Hull blackberries were purified using solid phase extraction to obtain anthocyanin-rich methanol fractions. This method concentrated phenolics and anthocyanins, recovering 97% and 76% of the total yield in puree or powder extracts, respectively, which represented a 24-63 fold increase of the total antioxidant capacity when compared with either the water fraction or the original extract. The ability of these fractions to protect primary keratinocytes against UV-induced oxidative damage was assessed. Anthocyanin-rich methanol fractions derived from either blackberry powder or puree exhibited strong antioxidant properties, protecting against UV-induced ROS nearly as efficiently as N-acetyl cysteine. Furthermore, the fractions up-regulated the expression of catalase, MnSOD, Gpx1/2 and Gsta1 antioxidant enzymes. Thus, it is concluded that blackberry extracts may protect keratinocytes against UV-mediated oxidative damage. Copyright (C) 2011 John Wiley & Sons, Ltd.
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