Journal
PHYTOTHERAPY RESEARCH
Volume 25, Issue 7, Pages 1011-1017Publisher
WILEY
DOI: 10.1002/ptr.3387
Keywords
avicularin; hepatoprotective effect; hirsutrin; Lespedeza cuneata; quercetin; radical scavenging activity
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Funding
- Ministry of Knowledge Economy [RTI05-01-02]
- Korea Institute of Industrial Technology(KITECH) [RTI05-01-02] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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The aerial parts of Lespedeza cuneata G. Don, perennial legume native to Eastern Asia, have been used therapeutically in traditional Asian medicine to protect the function of liver, kidneys and lungs. However, little is known about the pharmaceutical effect of extracts from this plant. In the present study, the aerial parts of L. cuneata were used to prepare an ethanol extract, which was then tested for hepatoprotective effects against injury by tert-butyl hyperoxide (t-BHP). At a dose of 20 mu g/mL, the ethanol extract significantly protected HepG2 cells against the cytotoxicity of t-BHP. Further fractionation of the extract with ethyl acetate allowed the isolation of five flavonoid compounds that were structurally identified by H-1 and C-13 NMR spectroscopy as isovitexin, hirsutrin, trifolin, avicularin and quercetin. Hirsutrin, avicularin and quercetin (10 mu M) showed clear hepatoprotective activity against injury by t-BHP in HepG2 cells, whereas isovitexin and trifolin showed no protective effects. The observed hepatoprotective effect of the investigated compounds showed a high correlation with radical scavenging activity, which followed the structure-activity relationships of the flavonoid aglycones. Copyright (C) 2011 John Wiley & Sons, Ltd.
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