Journal
PHYTOPATHOLOGY
Volume 103, Issue 6, Pages 594-599Publisher
AMER PHYTOPATHOLOGICAL SOC
DOI: 10.1094/PHYTO-10-12-0260-R
Keywords
broad-spectrum resistance
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Funding
- National Basic Research Program of China [2011CB100706, 2012CB114005]
- National Transgenic Research Project [2008ZX08009-001, 2008ZX08009-003, 2009ZX08001-011B]
- National Science Fund for Distinguished Young Scholars of China [31025017]
- National Natural Science Foundation of China [31160223]
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The rice blast resistance gene Pid3 encodes a nucleotide-binding-site leucine-rich repeat (NBS-LRR) protein. This gene was cloned from the rice 'Digu' (indica) by performing a genome-wide comparison of the NBS-LRR gene family between two genome-sequenced varieties, '9311' (indica) and 'Nipponbare' (japonica). In this study, we performed functional analysis of Pid3-A4, an ortholog of Pid3 revealed by allele mining in the common wild rice A4 (Oryza rufipogon). The predicted protein encoded by Pid3-A4 shares 99.03% sequence identity with Pid3, with only nine amino-acid substitutions. In wild rice plants, Pid3-A4 is constitutively expressed, and its expression is not induced by Magnaporthe oryzae isolate Zhong-10-8-14 infection. Importantly, in transgenic plants, Pid3-A4, as compared with Pid3, displays a distinct resistance spectrum to a set of M. oryzae isolates, including those that prevail in the rice fields of Sichuan Province. Therefore, Pid3-A4 should be quite useful for the breeding of rice blast resistance, especially in southwestern China.
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