Attenuation of isoproterenol-induced cardiotoxicity in rats by Narirutin rich fraction from grape fruit

Background: Oxidative stress is one of the major mechanism involved in pathogenesis of myocardial infarction. Use of natural products as therapeutic approach for ischemic myocardial injury is gaining attention worldwide. Purpose: This study was designed to investigate efficacy of Narirutin rich fraction (NRF), obtained from grape fruit peel, in the treatment of isoproterenol induced myocardial infarction in rats. Methods: After 3-days pretreatment with NRF (100 mg/kg and 200 mg/kg, p. o.) myocardial injury was induced by subcutaneous administration of isoproterenol (85 mg/kg) for 2 days. Hemodynamic parameters, biochemical parameters, histological and ultrastructural changes were observed. Results: Isoproterenol induced myocardial injury was evidenced by significant alterations in ECG, mean arterial pressure and left ventricular functions. Myocardial creatine kinase-MB isoenzyme, lactate dehydrogenase, superoxide dismutase, catalase, and glutathione level were reduced while MDE levels were increased. Histological findings also showed severe changes. Treatment with NRF significantly attenuated these parameters in dose dependent manner. Conclusion: Thus, present study provides evidences for efficacy of NRF against isoproterenol induced myocardial infarction in rats.